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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
09/01/2024 |
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Data da última atualização: |
09/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GEROMEL, C.; FERREIRA, L. P.; GUERREIRO, S. M. C.; CAVALARI, A. A.; POT, D.; PEREIRA, L. F. P.; LEROY, T.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. |
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Afiliação: |
CLARA GEROMEL, UNIVERSIDADE ESTADUAL DE CAMPINAS; LÚCIA PIRES FERREIRA, INSTITUTO AGRONÔMICO DO PARANÁ; SANDRA MARIA CARMELO GUERREIRO, UNIVERSIDADE ESTADUAL DE CAMPINAS; ALINE ANDRÉIA CAVALARI, UNIVERSIDADE ESTADUAL DE CAMPINAS; DAVID POT, INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, CNPCa; THIERRY LEROY, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRONÔMICA PARA O DESENVOLVIMENTO; LUIZ GONZAGA ESTEVES VIEIRA, INSTITUTO AGRONÔMICO DO PARANÁ; PAULO MAZZAFERA, UNIVERSIDADE ESTADUAL DE CAMPINAS; PIERRE MARRACCINI, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRONÔMICA PARA O DESENVOLVIMENTO. |
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Título: |
Biochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit development. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Journal of Experimental Botany, v. 57, n. 12, p. 3243-3258, 2006. |
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DOI: |
https://www.doi.org/10.1093/jxb/erl084 |
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Idioma: |
Inglês |
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Conteúdo: |
Sucrose metabolism and the role of sucrose synthase were investigated in the fruit tissues (pericarp, perisperm, and endosperm) of Coffea arabica during development. Acid invertase, sucrose phosphate synthase, and sucrose synthase activities were monitored and compared with the levels of sucrose and reducing sugars. Among these enzymes, sucrose synthase showed the highest activities during the last stage of endosperm and pericarp development and this activity paralleled closely the accumulation of sucrose in these tissues at this stage. Carbon partitioning in fruits was studied by pulse-chase experiments with C-14-sugars and revealed high rates of sucrose turnover in perisperm and endosperm tissues. Additional feeding experiments with (CO2)-C-14 showed that leaf photosynthesis contributed more to seed development than the pericarp in terms of photosynthate supply to the endosperm. Sugar analysis, feeding experiments, and histological studies indicated that the perisperm plays an important role in this downloading process. It was observed that the perisperm presents a transient accumulation of starch which is degraded as the seed develops. Two full-length cDNAs (CaSUS1 and CaSUS2) and the complete gene sequence of the latter were also isolated. They encode sucrose synthase isoforms that are phylogenetically distinct, indicating their involvement in different physiological functions during cherry development. Contrasting expression patterns were observed for CaSUS1 and CaSUS2 in perisperm, endosperm, and pericarp tissues: CaSUS1 mRNAs accumulated mainly during the early development of perisperm and endosperm, as well as during pericarp growing phases, whereas those of CaSUS2 paralleled sucrose synthase activity in the last weeks of pericarp and endosperm development. Taken together, these results indicate that sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit. MenosSucrose metabolism and the role of sucrose synthase were investigated in the fruit tissues (pericarp, perisperm, and endosperm) of Coffea arabica during development. Acid invertase, sucrose phosphate synthase, and sucrose synthase activities were monitored and compared with the levels of sucrose and reducing sugars. Among these enzymes, sucrose synthase showed the highest activities during the last stage of endosperm and pericarp development and this activity paralleled closely the accumulation of sucrose in these tissues at this stage. Carbon partitioning in fruits was studied by pulse-chase experiments with C-14-sugars and revealed high rates of sucrose turnover in perisperm and endosperm tissues. Additional feeding experiments with (CO2)-C-14 showed that leaf photosynthesis contributed more to seed development than the pericarp in terms of photosynthate supply to the endosperm. Sugar analysis, feeding experiments, and histological studies indicated that the perisperm plays an important role in this downloading process. It was observed that the perisperm presents a transient accumulation of starch which is degraded as the seed develops. Two full-length cDNAs (CaSUS1 and CaSUS2) and the complete gene sequence of the latter were also isolated. They encode sucrose synthase isoforms that are phylogenetically distinct, indicating their involvement in different physiological functions during cherry development. Contrasting expression patterns were observed for CaSUS1 and CaSUS2 ... Mostrar Tudo |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Endosperm; Gene expression; Sucrose synthase. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160617/1/Biochemical-genomic-analysis.pdf
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Marc: |
LEADER 02824naa a2200289 a 4500
001 2160617
005 2024-01-09
008 2006 bl uuuu u00u1 u #d
024 7 $ahttps://www.doi.org/10.1093/jxb/erl084$2DOI
100 1 $aGEROMEL, C.
245 $aBiochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit development.$h[electronic resource]
260 $c2006
520 $aSucrose metabolism and the role of sucrose synthase were investigated in the fruit tissues (pericarp, perisperm, and endosperm) of Coffea arabica during development. Acid invertase, sucrose phosphate synthase, and sucrose synthase activities were monitored and compared with the levels of sucrose and reducing sugars. Among these enzymes, sucrose synthase showed the highest activities during the last stage of endosperm and pericarp development and this activity paralleled closely the accumulation of sucrose in these tissues at this stage. Carbon partitioning in fruits was studied by pulse-chase experiments with C-14-sugars and revealed high rates of sucrose turnover in perisperm and endosperm tissues. Additional feeding experiments with (CO2)-C-14 showed that leaf photosynthesis contributed more to seed development than the pericarp in terms of photosynthate supply to the endosperm. Sugar analysis, feeding experiments, and histological studies indicated that the perisperm plays an important role in this downloading process. It was observed that the perisperm presents a transient accumulation of starch which is degraded as the seed develops. Two full-length cDNAs (CaSUS1 and CaSUS2) and the complete gene sequence of the latter were also isolated. They encode sucrose synthase isoforms that are phylogenetically distinct, indicating their involvement in different physiological functions during cherry development. Contrasting expression patterns were observed for CaSUS1 and CaSUS2 in perisperm, endosperm, and pericarp tissues: CaSUS1 mRNAs accumulated mainly during the early development of perisperm and endosperm, as well as during pericarp growing phases, whereas those of CaSUS2 paralleled sucrose synthase activity in the last weeks of pericarp and endosperm development. Taken together, these results indicate that sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit.
650 $aEndosperm
650 $aGene expression
650 $aSucrose synthase
650 $aCoffea Arábica
700 1 $aFERREIRA, L. P.
700 1 $aGUERREIRO, S. M. C.
700 1 $aCAVALARI, A. A.
700 1 $aPOT, D.
700 1 $aPEREIRA, L. F. P.
700 1 $aLEROY, T.
700 1 $aVIEIRA, L. G. E.
700 1 $aMAZZAFERA, P.
700 1 $aMARRACCINI, P.
773 $tJournal of Experimental Botany$gv. 57, n. 12, p. 3243-3258, 2006.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 11 | |
| 1. |  | GEROMEL, C.; MAZZAFERA, P.; MARRACCINI, P. R.; FERREIRA, L. P.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Açúcares solúveis, sacarose sintase e sacarose fosfato sintase durante o desenvolvimento do fruto de café, sob diferentes condições In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia. Anais... Brasília, DF: Embrapa Café, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
|    |
| 2. | | GEROMEL, C.; MAZZAFERA, P.; MARRACCINI, P. R.; FERREIRA, L. P.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Açúcares solúveis, sacarose sintase e sacarose fosfato sintase durante o desenvolvimento do fruto de café, sob diferentes condições de luz e carga. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 3. | | FERREIRA, L. P.; GEROMEL, C.; CAVALAR, A. A.; MARRACCINI, P.; MAZZAFERA, P.; PEREIRA, L. F. P.; MAZZAFERA, P. Caracterização molecular de enzimas chave do metabolismo de açúcares em café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 3., 2003, Porto Seguro. Resumos... Brasília, DF: Embrapa Café, 2003.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
| |
| 4. | | PINTO, J. E. B. P.; ABREU, I. N. de; GEROMEL, C.; SANTIAGO, E. J. A.; FIDELIS, I. In vivo and in vitro propagation, callus induction, mineral nutrition and quantification of mucilage in Cissus sicyoides, a medicinal plants. In Vitro Cellular & Developmental Biology - Animal, v. 35, n. 3, p. 53A, Mar. 1999. Contem resumo dos trabalhos apresentados no Congress on In Vitro Biology, 1999, New Orleans.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 5. | | GEROMEL, C.; FERREIRA, L. P.; GUERREIRO, S. M. C.; CAVALARI, A. A.; POT, D.; PEREIRA, L. F. P.; LEROY, T.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. Biochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit development. Journal of Experimental Botany, v. 57, n. 12, p. 3243-3258, 2006.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Café. |
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| 6. | | GEROMEL, C.; PEREIRA, L. P.; CAVALARI, A. A.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; LEROY, T.; MAZZAFERA, P.; MARRACCINI, P. Metabolismo de açúcares durante o desenvolvimento de frutos de café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
| |
| 7. | | ABREU, I. N. de; PINTO, J. E. B. P.; BERTOLUCCI, S. K. V.; MORAIS, A. R. de; GEROMEL, C.; LADEIRA, A.; LAMEIRA, O. A. Propagação in vivo e in vitro de Cissus sicyoides, uma planta medicinal. Acta Amazonica, Manaus, v. 33, n. 1, p. 1-7, jan./mar. 2003.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | GEROMEL, C.; FERREIRA, L. P.; BOTTCHER, A.; POT, D.; PEREIRA, L. F. P.; LEROY, T.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. Sucrose metabolism during fruit development in coffea racemosa. Annals of Applied Biology, v. 152, n. 2, p.179-187, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Café. |
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| 9. | | GEROMEL, C.; FERREIRA, L. P.; CAVALARI, A. A.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; LEROY, T.; MAZZAFERA, P.; MARRACCINI, P. Sugar metabolism during coffee fruit development. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 20., 2004, Bangalore, India. Anais... Montpellier, France: Association for Science and Information on Coffee, 2005. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 10. | | GEROMEL, C.; FERREIRA, L. P.; DAVRIEUX, F.; GUYOT, B.; RIBEYRE, F.; SCHOLZ, M. B. dos S.; PEREIRA, L. F. P.; VAAST, P.; POT, D.; LEROY, T.; ANDROCIOLI FILHO, A.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. Effects of shade on the development and sugar metabolism of coffee (Coffea arabica L.) fruits. PLANT PHYSIOLOGY AND BIOCHEMISTRY, v.46, n.5-6, p.569-579, may-june, 2008. 569-579| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Café. |
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| 11. | | MARRACCINI, P.; GEROMEL, C.; FERREIRA, L. P.; PEREIRA, L. F. P.; LEROY, T.; POT, D.; DAVRIEUX, F.; RIBEYRE, F.; GUYOT, B.; SCHOLZ, M. B. S.; ANDROCIOLI FILHO, A.; VIEIRA, L. G. E.; MAZZAFERA, P. Soluble sugars, enzymatic activities and gene expression during development of coffee fruits submitted to shade condition. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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